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Title: Comparison of drug sensitivity and pathogenicity of Trypanosoma bruci rhodesiense isolates with their respective clones originating from Busia and Busoga
Authors: Korir, Scolastica,Chepngetich
Keywords: Drug sensitivity
Issue Date: Oct-2011
Publisher: Egerton University
Abstract: Human Afiican trypanosomosis (sleeping sickness) is a protozoan disease caused by trypanosome parasites transmitted by tsetse flies (vectors) through bites. It is found mostly in Afiica where about 60 million people are at risk. Studies using clones in the laboratory show variations in pathogenicity and drug sensitivity compared to their respective natural isolates for various trypanosome species. This suggests that within isolates there exist sub-populations due to change overtime. The objective of this study was to characterize T13/panosoma brucei rhodesiense isolated fi'on1 patients in two different sleeping sickness foci, (Busoga and Busia) and evaluate the change in drug sensitivity and pathogenicity with time of isolation. For this study, various T rypanosoma bmcei rhodesiense clones and their respective isolates were tested for pathogenicity and sensitivity to drugs (Suramin and Mel B) in Swiss white mice. The clinical, pathological and sensitivity parameters were determined and the quantitative data were statistically analyzed using standard methods. Results showed that when compared with the specific isolates, clones had lower mean pre-patent periods and lower mean post infection survival periods. Busia KETRI 3800 and Busoga KETRI 3380 clones extracted from isolates collected in 2000 and 1976 respectively appeared most pathogenic with the lowest mean survival periods of 20 and Zldays respectively. Furthermore, when compared with the controls, PCV changes for all the clones were significantly different (p<0.05) but this was not the case for the isolates. Clinical signs were more pronotmced in mice infected with clones than those infected with the isolates. Mice infected with isolates fiom Busia and Busoga had no significant differences (p>0.05) in their PCV and bodyweights. However, mice infected with isolates collected earlier (in 1987 and 1971 respectively) recorded lower PCV and survived for a shorter period. On the other hand, Busoga isolates (EATRO 2285 and EATRO 1886) appeared chronic with the highest survival periods, of 44 and 47 days respectively. Some infected non treated mice although weak, with low PCV and very high parasitaemia were live by the time the experiment was terminated. This study concluded that the clones were more pathogenic than their isolates. Drug sensitivity results for various isolates and their respective clones were comparable at all dosages except for EATRO 1886 isolate which was found to be resistant to 2.5mg/kg of Suramin. Age of the isolate played an important role in the pathological variations observed between isolates of the same area.
Appears in Collections:Faculty of Veterinary Medicine and Surgery

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