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Title: Molecular Classification of Kenyan Wheat Varieties
Authors: Amana, Mzee Juma
Keywords: Molecular Classification - Wheat Varieties
Issue Date: Nov-2008
Publisher: Egerton University
Abstract: Wheat cultivars in Kenya have been classified using traditional methods, which are based on their dough and morphological characteristics. The methods have numerous drawbacks and among others they lack repeatability and are unreliable. It is, therefore essential to develop a reliable and precise method for classifying wheat cultivars. In this study, thirty (30) Kenyan wheat varieties have been classified using molecular based markers based on gliadins, high and low molecular weight glutenin subunits and albumins. The study provides a fairly easy to use tool for description of wheat varieties virtually unaffected by agronomic practices. The varieties were evaluated for the above molecular markers using Soditun dodecyl sulphate — polyacrylamide gel electrophoresis (SDS — PAGE). Genetic trees were developed using Phylip based on allele combination of the markers in the varieties. All the markers evaluated gave different groupings. based on the high molecular weight — glutenin subunits (HMW-GS) and quality of the wheat, the test cultivars were classified into two groups. The first group which comprised wheat varieties with strong baking quality had the allele combinations 5+l0, while the group with weak baking quality had alleles 2+l2. The thirty wheat varieties were also classified using Low molecular weight — glutenin subunits (LMW-GS), Gliadins and albumins from the genetic distances generated from the allele combinations. All markers were also used to generate a genetic tree and group 2 of the classification had varieties which are suited to an altitude range of between l800m — 2400m. This type of classification has provided a database of allele profiles that will be useful in planning more effective wheat breeding strategies, quality control, classification of newly released cultivars and for further research in this field. Due to their high level of polymorphism, the markers can also be useful in varietal identification. Random amplified polymorphic DNA (RAPD) and Inter- simple sequence repeat (ISSR) — polymerase chain reaction (PCR) based markers were also assayed in the test germplasm though they failed to reveal polymorphism among assayed samples. Further research on the use of these molecular markers is recommended.
Appears in Collections:Faculty of Science

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