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Catechols in caffeic acid phenethyl ester are essential for inhibition of TNF-mediated IP-10 expression through NF-jB-dependent but HO-1- and p38-independent mechanisms in mouse intestinal epithelial cells

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dc.contributor.author Mapesa, Job O.
dc.contributor.author Waldschmitt, Nadine
dc.contributor.author Schmoeller, Ingrid
dc.contributor.author Blume, Carolin
dc.contributor.author Hofmann3, Thomas
dc.contributor.author Mahungu, Symon
dc.contributor.author Clavel, Thomas
dc.contributor.author Haller, Dirk
dc.date.issued 2012
dc.date.accessioned 2021-05-17T07:23:39Z
dc.date.available 2021-05-17T07:23:39Z
dc.identifier.uri http://41.89.96.81:8080/xmlui/handle/123456789/2530
dc.description.abstract Scope: Caffeic acid phenethyl ester (CAPE) is an active constituent of honeybee propolis inhi- biting nuclear factor (NF)-kB. The aims of our study were to provide new data on the functional relevance and mechanisms underlying the role of CAPE in regulating inflammatory processes at the epithelial interface in the gut and to determine the structure/activity relationship of CAPE. Methods and results: CAPE significantly inhibited TNF-induced IP-10 expression in intestinal epithelial cells. Using various analogues, we demonstrated that substitution of catechol hydroxyl groups and addition of one extra hydroxyl group on ring B reversed the functional activity of CAPE to inhibit IP-10 production. The anti-inflammatory potential of CAPE was confirmed in ileal tissue explants and embryonic fibroblasts derived from TNFDARE/1 mice. Interestingly, CAPE inhibited both TNF- and LPS-induced IP-10 production in a dose- dependent manner, independently of p38 MAPK, HO-1 and Nrf2 signaling pathways. We found that CAPE did not inhibit TNF-induced IkB phosphorylation/degradation or nuclear translocation of RelA/p65, but targeted downstream signaling events at the level of tran- scription factor recruitment to the gene promoter. Conclusion: This study reveals the structure–activity effects and anti-inflammatory potential of CAPE in the intestinal epithelium. en_US
dc.language.iso en en_US
dc.publisher Egerton University en_US
dc.title Catechols in caffeic acid phenethyl ester are essential for inhibition of TNF-mediated IP-10 expression through NF-jB-dependent but HO-1- and p38-independent mechanisms in mouse intestinal epithelial cells en_US
dc.type Other en_US


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