dc.contributor.author |
Maritim, Winnie ,Cherotich |
|
dc.contributor.author |
Wagara, Isabel ,Nyokabi |
|
dc.contributor.author |
Matasyoh, Josphat, Clement |
|
dc.date.issued |
2019-05 |
|
dc.date.accessioned |
2024-01-31T08:31:14Z |
|
dc.date.available |
2024-01-31T08:31:14Z |
|
dc.identifier.uri |
https://www.researchgate.net/publication/345448303_Antibacterial_saponins_from_the_leaves_of_Polyscias_fulva_Araliaceae/link/5fa6fe9c299bf10f732d3822/download |
|
dc.description.abstract |
Saponins are a major family of secondary metabolites that occur in a wide range of plant species.
Bioassay - guided fractionation of extract of the leaves of Polyscias fulva led to the isolation of three
known saponins named, 3-O-α-L-arabinopyranosyl-hederagenin (1), 3-O-[α-L-rhamnopyranosyl(1-2)-α-L-
arabinopyranosyl]-hederagenin (2) and 3-O-[rhamnopyranosyl-(1→2)-xylopyranosyl]-Olean-12-en-28-O-
[rhamnopyranosyl-(1→4)-glucopyranosyl-(1→6-glucopyranosyl] ester) (3). Leaves of the plant were
collected from Kakamega rain forest in Kenya, dried under shade and ground into fine powder and
extraction was done using methanol. The methanol extract was subjected to column chromatography
and the fractions purified using preparative high performance liquid chromatography (HPLC). The
bioactivity of the pure compounds was done using disc diffusion method. The three compounds
exhibited moderate activities against Gram positive bacterium (Staphylococcus aureus ATCC25922)
and Gram negative bacterium (Klebsiella pneumoniae ATCC13883). Compound 1 was found to be the
most active against K. pneumoniae (8.00±1.00 mm) and S. aureus (10.00±1.73 mm) followed by
compound 2 with inhibition zones of 7.66±0.57 and 7.33±0.57 mm against K. pneumoniae and S. aureus,
respectively. Compound 3 was the least active against both K. pneumoniae (7.33±0.57 mm) and S.
aureus (7.00±1.00 mm). The results obtained indicate that compounds 1, 2 and 3 exhibit potential as
possible sources of antibacterial agents. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
African Journal of Biotechnology |
en_US |
dc.subject |
Antibiotics |
en_US |
dc.subject |
Bacterial infections |
en_US |
dc.title |
Antibacterial saponins from the leaves of Polyscias fulva (Araliaceae) |
en_US |
dc.type |
Article |
en_US |